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Mr Cameron said he could "absolutely" give a cast-iron guarantee that the vote would go ahead in 2017 if he returned to Downing Street and that he would stand down if he could not promise a referendum in a future coalition.
"I’ve said very clearly that whatever the outcome of the next election, and of course I want an overall majority, and I’m hoping and believing I can win an overall majority, but people should be in no doubt I will not become prime minister unless I can guarantee that we will hold that referendum", he said.
"I think it is essential that... the treaties make clear that [that clause] doesn’t apply to Britain", the prime minister said. "Some of the things I’m calling for will require treaty change ... why this is so important is that there is a sense in this country, which I understand and in many ways share, that we wanted to be part of a single market, where nation states co-operate; we did not want to be part of a country called Europe – our country is United Kingdom."
However, the prime minister did pull back from opposing free movement within the EU – an issue which has provoked significant concern among Conservative Eurosceptics. He said that rather than withdrawing from this right, the UK would seek to return free movement to its core purpose.